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KMID : 1144820220280040298
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대한의생명과학회지
2022 Volume.28 No. 4 p.298 ~ p.306
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Comparative Efficacy of Various Formalin Fixatives for Molecular Diagnosis in Pathological Tissues
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Jee Woo-hyun
Bae Moon-hwan
Yoon Hye-Jin
Kang In-young
Koo Myoung-joo
Lee Jae-wang
Jun Jin-Hyun
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Abstract
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Pathological tissue fixation using formalin has been widely used for histological samples in many hospitals and institutions. In general, formalin fixatives were either manufactured in laboratories or purchased commercially because of the risks and environmental concerns of handling organic compounds. In this study, the efficacy of three kinds of commercially purchased and one laboratory-made formalin fixative was compared in the PCR-based molecular diagnosis using the extracted DNA from formalin-fixed paraffin-embedded (FFPE) tissues. The quality of extracted DNA from FFPE tonsil tissues with four kinds of formalin solutions was evaluated, and PCR for beta-globin gene and microsatellite instabilities (MSI) tests for pentaplex panel markers were performed using the extracted DNA. There was no difference in PCR and MSI tests as molecular diagnoses regardless of the types of formalin used in this study. However, the total amount and average length of double-stranded DNA extracted from FFPE tonsil tissue showed significant differences according to the type of formalin fixative. Optimized formalin fixatives and methods for DNA extraction might be sophisticated to extract good quality DNA from the small size of specific tissue samples. Further studies are needed to select the most effective formalin fixative for histology and molecular pathology using human FFPE tissues.
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KEYWORD
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Formalin, Formalin-fixed paraffin embedded (FFPE) tissues, Histology, Molecular diagnosis, DNA extraction, Polymerase chain reaction (PCR), microsatellite instabilities (MSI) tests
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